反应详情

13.7 mg (325 μmol) of lithium hydroxide monohydrate are added to a solution of 19 mg (32.5 μmol) of methyl 4-[(4E)-3-[4-(methoxycarbonyl)benzyl]-5-{2-[4-(2-oxopyrrolidin-1-yl)butoxy]-phenyl}pent-4-en-1-yl]benzoate (Example 36A) in 0.5 ml of dioxane and 0.5 ml of 2 M aqueous sodium hydroxide solution, and the mixture is stirred at room temperature for 16 h. The mixture is then adjusted to pH 2 using 1 M hydrochloric acid and extracted twice with ethyl acetate. The combined organic phases are dried over sodium sulfate, filtered and concentrated. The residue obtained is purified by preparative HPLC. This gives 1.8 mg (3.2 μmol, 10.0% of theory) of the title compound.

1. extractionextracted twice with ethyl acetate
2. dry with materialThe combined organic phases are dried over sodium sulfate
3. filtrationfiltered
4. concentrationconcentrated
5. customThe residue obtained
6. customis purified by preparative HPLC