反应详情

温度

-10 °C

To a cooled (cooling bath −10° C.) stirred suspension of sodium nitrite (82 mg, 1.2 mmol) in conc. HCl (2.6 mL) was added a solution of 3-(4-fluorophenyl)-1-(2-morpholinoethyl)-4-(phenylethynyl)-1H-pyrazol-5-amine (129 mg, 0.33 mmol) in trifluoroacetic acid (0.5 ml) and the reaction mixture was allowed to warm to ambient temperature over 2 h. Water and CH2Cl2 were added. The aqueous phase was extracted with CH2Cl2 (×3) and the organic phases combined, dried over MgSO4, filtered and evaporated. The crude material was purified by preparative HPLC to give 3-(4-fluorophenyl)-1-(2 -morpholinoethyl)-5-phenyl-1H-pyrazolo[3,4-c]pyridazin-4-ol as a solid (27.8 mg) which was used as such in the subsequent step.

1. temperatureTo a cooled
2. temperatureto warm to ambient temperature over 2 h
3. extractionThe aqueous phase was extracted with CH2Cl2 (×3)
4. dry with materialdried over MgSO4
5. filtrationfiltered
6. customevaporated
7. customThe crude material was purified by preparative HPLC