反应详情

温度

50 °C

Tetraacetylguanosine (0.904 g, 2.00 mmols) was dissolved in 2 ml of acetonitrile, and 0.59 ml (2.48 equivalents) of benzyl bromide were added thereto. The reaction was conducted at 50° C. for 17 hours. After it was identified through HPLC that the starting material disappeared, 5 ml (29.6 equivalents) of conc. hydrochloric acid were added to the reaction solution, and the mixture was stirred at 50° C. for 3 hours. Then, the reaction solution turned black, and the gray solid was precipitated. The temperature was returned to room temperature, and 5 ml of methanol were added to the solid. The slurry was stirred for 20 minutes, and then filtered. The crystals were washed with 10 ml of methanol, and dried at 50° C. under reduced pressure to give 0.690 g of gray crystals in a yield of 57.4%.

1. customthe gray solid was precipitated
2. customwas returned to room temperature
3. addition5 ml of methanol were added to the solid
4. stirringThe slurry was stirred for 20 minutes
5. filtrationfiltered
6. washThe crystals were washed with 10 ml of methanol
7. customdried at 50° C. under reduced pressure