反应详情

Methanesulfonyl chloride (25 μl) and triethylamine (46 μl) were added to a solution of H-Val-Apns-Dmt-NHBzl(2-Me) (162 mg) in methylene chloride (3 ml) and the mixture was stirred for two hours. After the addition of ethyl acetate, the reaction mixture was washed with 3% Na2CO3, 1N HCl, and 5% NaCl and dried over MgSO4. After filtration and concentration, the residue was purified using silica gel column chromatography and reprecipitated from ethyl acetate/n-hexane to obtain the title compound (127 mg).

1. washthe reaction mixture was washed with 3% Na2CO3, 1N HCl, and 5% NaCl
2. dry with materialdried over MgSO4
3. filtrationAfter filtration and concentration
4. customthe residue was purified
5. customreprecipitated from ethyl acetate/n-hexane