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HRID1606394

反应详情

EQUATION

反应方程式

HRID 1606394 的结构方程式

REACTANTS

反应物

1
结构：NC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H](N)C[C@@H]2N)[C@H](O)[C@@H](O)[C@@H]1O.O=S(=O)(O)O
HCID441374

Kanamycin A sulfate

C18H38N4O15S

PRODUCTS

生成物

2
结构：NC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H](N)C[C@@H]2N)[C@H](O)[C@@H](O)[C@@H]1O
HCID6032

Kanamycin A

C18H36N4O11

结构：NC[C@H]1O[C@H](O[C@H]2[C@H](O)[C@@H](O[C@H]3O[C@H](CO)[C@@H](O)[C@H](N)[C@H]3O)[C@H](N)C[C@@H]2N)[C@H](O)[C@@H](O)[C@@H]1O.O=S(=O)(O)O
HCID441374

Kanamycin A sulfate

C18H38N4O15S

收率 186%

AUXILIARIES

试剂、催化剂与溶剂

1
结构：O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
溶剂HCID107526

未命名化合物

CONDITIONS

反应条件

温度
120 °C

PROCEDURE

实验过程

Simulated kanamycin culture broth is prepared by adding 80 mg of kanamycin sulfate to 160 ml of a glucose bouillon medium, which s sterilized under heating at 120°C for 15 minutes and cooled. The culture broth is then passed through a column of 1.5 cm in diameter packed with 30 g of the selective adsorbing particles obtained in Example 2 at a flowing velocity of 0.5 ml/minute. The column is then washed with water and further eluted with 0.01 N HCl-methanol (1 : 1). Respective active fractions are combined to give the result that 74.4 mg (recovery percentage: 91%) of kanamycin sulfate is obtained in very high purity and yield with 98% decoloration degree and 98% purity.

WORKUP

后处理

  1. temperaturecooled
  2. customobtained in Example 2 at a flowing velocity of 0.5 ml/minute
  3. washThe column is then washed with water
  4. washfurther eluted with 0.01 N HCl-methanol (1 : 1)
  5. customto give the