Phosphoenolpyruvate → Succinic Acid

HRID 1641990Notes

Reaction equation

[C:1]([OH:8])(=[O:7])[CH2:2][CH2:3][C:4]([OH:6])=[O:5].C=C(OP(O)(O)=O)C(O)=O.C([O-])(=O)C(CC([O-])=O)O.C([O-])(=O)C=O.[O:33]=[CH:34][C@@H:35]([C@H:37]([C@@H:39]([C@@H:41]([CH2:43][OH:44])[OH:42])[OH:40])[OH:38])[OH:36]>>[OH:44][CH2:43][C@@H:41]([C@H:39]([C@@H:37]([C@@H:35]([CH2:34][OH:33])[OH:36])[OH:38])[OH:40])[OH:42].[C:1]([OH:8])(=[O:7])[CH2:2][CH2:3][C:4]([OH:6])=[O:5]

Participants

Reactant 5

HCID 1005Phosphoenolpyruvate2×

HCID 1110Succinic Acid4×

HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal2×

HCID 160434DL-Malate

HCID 3614358Npc324351

Product 2

HCID 1110Succinic Acid

HCID 5780Sorbitol

Procedure

Metabolic flux analysis was used to evaluate the effect of different carbon sources on succinic acid production in batch fermentations with A. succinogenes FZ45. The analyses established that the major pathway for succinic acid production in A. succinogenes FZ45 flows in the following manner: phosphoenolpyruvate (PEP)→oxaloacetate (OAA)→malate→fumarate→succinic acid. The glyoxylate shunt and the PEP-transport-system (PTS) appear not to be substantially used in the organism. Glucose fermentations reach a concentration of 61.7 g/L succinic acid with a yield of about 94% (succinic acid (g)/glucose (g)). Fermentations performed using a more reduced carbon source, such as sorbitol, produced higher amounts of succinic acid (77.3 g/L) with a higher yield (108% succinic acid (g)/glucose (g)), indicating that reducing power may become a limiting factor during the fermentation of glucose.

Sources & evidence

ORD record: ord-ad431c0de55341bfbdc87511305a3a33
Source dataset: uspto-grants-2012_02
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US08119377B2

Reaction notes

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