反应详情

70 mg (0.17 mmol) of 4-(2-amino-6-{[3-fluoro-4-(1H-pyrrolo[2,3-b]pyridin-4-yloxy)phenyl]amino}pyrimidin-4-yl)butyric acid (from example 130) are dissolved in 2.0 ml of DMF, 60 mg (0.16 mmol) of HATU, 23 mg (0.17 mmol) of 1-hydroxy-1H-azobenzotriazole hydrate, 43 mg (0.33 mmol) of diisopropylamine and 28 mg (0.33 mmol) of piperidine are added and the mixture is stirred at RT for 20 hours. Volatile components are removed under reduced pressure and the product is purified by preparative HPLC. The resulting product is purified further by thick-layer chromatography (1 mm silica gel coating, mobile phase: DCM:methanol=9:1).

1. customVolatile components are removed under reduced pressure
2. customthe product is purified by preparative HPLC
3. customThe resulting product is purified further by thick-layer chromatography (1 mm silica gel coating, mobile phase: DCM:methanol=9:1)