反应详情

To a stirred mixture of (S)-2-amino-3-(biphenyl-4-yl)-propionic acid benzyl ester hydrochloride (4.7 g, 12.8 mmol) in ethyl acetate (100 mL) and water (100 mL) at 5° is added sodium bicarbonate (1.13 g, 13.5 mmol). The solution is stirred for 5 minutes, then aqueous formaldehyde (37% aq., 1.25 mL, 16.6 mmol) is added. The mixture is allowed to warm slowly to room temperature while being stirred vigorously for 18 hours. The organic layer is separated and the aqueous layer extracted with ethyl acetate (50 mL). The combined organic phases are washed with water (50 mL), dried over anhydrous sodium sulfate, filtered and concentrated in vacuo. The resulting crude hexahydrotriazine intermediate is obtained as a white solid that is dried under high vacuum. Some of that material (0.75 g, 2.18 mmol) is dissolved in toluene (10 mL) and kept under nitrogen. Diphenyl phosphite (0.5 mL, 2.62 mmol) is added and the solution is warmed in a bath pre-heated to 70°. After 2 hours, the reaction mixture is allowed to cool to room temperature. After evaporation of the solvent under reduced pressure, the residue is purified by flash-chromatography on silica gel, eluting with a gradient of ethyl acetate (50% to 90%) in hexane. The product is recrystallized from ether/hexane, affording (S)-3-(biphenyl-4-yl)-2-[(diphenylphosphonomethyl)-amino]-propionic acid benzyl ester as a while solid, m.p. 85°-86°; [α]D =+8.40 (c 1.04, CHCl3).

1. stirringwhile being stirred vigorously for 18 hours
2. customThe organic layer is separated
3. extractionthe aqueous layer extracted with ethyl acetate (50 mL)
4. washThe combined organic phases are washed with water (50 mL)
5. dry with materialdried over anhydrous sodium sulfate
6. filtrationfiltered
7. concentrationconcentrated in vacuo
8. customThe resulting crude hexahydrotriazine intermediate is obtained as a white solid
9. customthat is dried under high vacuum
10. temperaturethe solution is warmed in a bath
11. temperaturepre-heated to 70°
12. waitAfter 2 hours
13. temperatureto cool to room temperature
14. customAfter evaporation of the solvent under reduced pressure
15. customthe residue is purified by flash-chromatography on silica gel
16. washeluting with a gradient of ethyl acetate (50% to 90%) in hexane
17. customThe product is recrystallized from ether/hexane