1-Hydroxybenzotriazole → ((S)-1-Cyclohexyl-2-{(S)-2-[2-(1H-indol-3-yl)-pyridin-4-yl]-pyrrolidin-1-yl}-2-oxo-ethyl)-carbamic acid tert-butyl ester

HRID 1667626Notes

Reaction equation

HRID 1667626 reaction equation
[C:1]([O:5][C:6]([NH:8][C@@H:9]([CH:13]1[CH2:18][CH2:17][CH2:16][CH2:15][CH2:14]1)[C:10]([OH:12])=O)=[O:7])([CH3:4])([CH3:3])[CH3:2].C1C=CC2N(O)N=NC=2C=1.CN(C(ON1N=NC2C=CC=CC1=2)=[N+](C)C)C.F[P-](F)(F)(F)(F)F.[NH:53]1[CH2:57][CH2:56][CH2:55][C@H:54]1[C:58]1[CH:63]=[CH:62][N:61]=[C:60]([C:64]2[C:72]3[C:67](=[CH:68][CH:69]=[CH:70][CH:71]=3)[NH:66][CH:65]=2)[CH:59]=1.C(N(C(C)C)CC)(C)C>CN(C=O)C.O>[C:1]([O:5][C:6](=[O:7])[NH:8][C@@H:9]([CH:13]1[CH2:18][CH2:17][CH2:16][CH2:15][CH2:14]1)[C:10]([N:53]1[CH2:57][CH2:56][CH2:55][C@H:54]1[C:58]1[CH:63]=[CH:62][N:61]=[C:60]([C:64]2[C:72]3[C:67](=[CH:68][CH:69]=[CH:70][CH:71]=3)[NH:66][CH:65]=2)[CH:59]=1)=[O:12])([CH3:2])([CH3:3])[CH3:4]

Participants

8

Reactant 5

HCID 757711-HydroxybenzotriazoleHCID 81531DiisopropylethylamineHCID 2733084Methanaminium, N-((1H-benzotriazol-1-yloxy)(dimethylamino)methylene)-N-methyl-, hexafluorophosphate(1-) (1:1)HCID 7004938N-(tert-Butoxycarbonyl)-L-cyclohexylglycineHCID 668898983-((S)-4-pyrrolidin-2-yl-pyridine-2-yl)-1H-indole

Product 1

HCID 66889931((S)-1-Cyclohexyl-2-{(S)-2-[2-(1H-indol-3-yl)-pyridin-4-yl]-pyrrolidin-1-yl}-2-oxo-ethyl)-carbamic acid tert-butyl esterYield 78%

Reagents, catalysts & solvents 2

HCID 962WaterSolventHCID 6228DimethylformamideSolvent2×

Procedure

A solution of (S)-tert-butoxycarbonylamino-cyclohexyl-acetic acid (75 mg, 0.29 mmol), HOBt (46 mg, 0.33 mmol) and HBTU (127 mg, 0.33 mmol) in DMF (5 mL) is stirred at room temperature for 30 min. Then a solution of 3-((S)-4-pyrrolidin-2-yl-pyridine-2-yl)-1H-indole (15) in DMF (5 mL) is added, followed by diisopropylethylamine (198 mg, 1.50 mmol). After stirring at room temperature for 2 h, the reaction mixture is diluted with water and extracted with EtOAc. The combined organic layers are washed with water, sat. NaHCO3, brine, dried over Na2SO4, filtered and concentrated down. The crude product is purified by flash chromatography on silica gel (EtOAc/Hexane: 5%˜40%) to give ((S)-1-Cyclohexyl-2-{(S)-2-[2-(1H-indol-3-yl)-pyridin-4-yl]-pyrrolidin-1-yl}-2-oxo-ethyl)-carbamic acid tert-butyl ester as a yellow solid (120 mg, 78%). Mass M/Z=503.34 [M+1]

Workup

  1. extractionextracted with EtOAc
  2. washThe combined organic layers are washed with water, sat. NaHCO3, brine
  3. dry with materialdried over Na2SO4
  4. filtrationfiltered
  5. concentrationconcentrated down
  6. customThe crude product is purified by flash chromatography on silica gel (EtOAc/Hexane: 5%˜40%)

Sources & evidence

ORD record
ord-5492259a1dd84d26831ae4aef8053ab8
Source dataset
uspto-grants-2011_10
Patent number
US08044209B2

Reaction notes

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