反应详情

A solution of compound 13 (1.0 equiv., 0.207 g, 0.45 mmol) in a mixture of DMF (5 ml) and concentrated aqueous HCl (10 ml) was heated at reflux for 2 days. After cooling to room temperature, the reaction product was filtered off and washed with isopropanol and isopropylether successively to give the hydrochloride salt of 2-[[5,6-dihydro-6-(4-nitrophenyl)-5-oxo-pyrido[2′,1′:2,3]imidazo[4,5-c]isoquinolin-9-yl]oxy]-acetic acid (14) (0.165 g, yield=96%, purity (LC)=90%) as a light brown powder.

1. temperaturewas heated
2. temperatureat reflux for 2 days
3. filtrationthe reaction product was filtered off
4. washwashed with isopropanol and isopropylether successively