反应详情

100 ml of a modified Czapek's liquid culture medium (consisting of glucose 30 g, magnesium sulfate 0.5 g, sodium nitrate 2 g, potassium dihydrogenphosphate 1 g, iron sulfate 0.01 g and deionized water 1000 ml) was put into a 200 ml.-capacity flask, which was sterilized at 115°C. for 15 minutes. The resultant culture medium was inoculated with a pieceof the growth (punched out with a cork borer, diameter 1 cm) of Rhizoctoniasolani AG-1 (accession number IFO-30465) cultured on a potato sucrose agar (PSA) culture medium at 28° C. for two days, which was subjected toshake-culture at 28° C. for four days. Then the same experiment was carried out with the exception that validamycin A was added to the culturemedium before the cultivating the medium, the amount of validamycin A being0.005% relative to the whole culture medium. In each of the two experiments, the culture cells obtained thus above were respectively separated and purified, followed by subjecting to quantitative determination. As shown in Table 1, from the culture cells by culturing inthe medium to which validamycin A was supplemented, a remarkably increased amount of trehalose was obtained as compared with those by culturing in the medium to which no validamycin A was supplemented.

1. customat 28° C.
2. customfor four days
3. additionwas added to the culturemedium before the cultivating the medium
4. customIn each of the two experiments, the culture cells obtained thus above
5. customwere respectively separated
6. custompurified