反应详情

To 0.57 g (3 mmol) of para-amino-benzenesulfonyl azide dissolved in 5 mL dimethylformamide was added 0.9 mL (6 mmol) triethylamine, then the (2,2,2-trifluro-acetylamino)-hexanoyl chloride dissolved in 10 mL dimethylformamide was added dropwise. The mixture turned brown and was stirred under argon for 24 hrs. Solvent was evaporated, and the remaining oil was dissolved in 10 mL dichloromethane and left at room temperature for 1 hr. The precipitate was filtrated and the solution was evaporated to yield 1.10 g crude oil. The obtained compound was directly used in oligonucleotide synthesis.

1. additionwas added dropwise
2. customSolvent was evaporated
3. dissolutionthe remaining oil was dissolved in 10 mL dichloromethane
4. waitleft at room temperature for 1 hr
5. filtrationThe precipitate was filtrated
6. customthe solution was evaporated