Water → (2S)-2-(carbamoylamino)-4-(methylsulfanyl)butanoic acid

Mixtures of (1) 2.0 ml. of an aqueous substrate solution prepared by dissolving DL-5-(2-methylthioethyl)hydantoin in a 0.1 M NH4Cl-NH4OH buffer solution of pH 9.5 (substrate concentration: 2.0%) and (2) 2.0 ml. of the above cell suspension were prepared and placed in test tubes, respectively. The hydrolysis reaction was then carried out at 30° C. for one hour without shaking. Immediately after the completion of the reaction, 1.0 ml. of a 10% aqueous solution of trichloroacetic acid, 1.0 ml. of a 10% solution of p-dimethylaminobenzaldehyde in 6 N hydrochloric acid and 6.0 ml. of distilled water were added to each reaction mixture. Each resulting color-developed reaction mixture was centrifuged to remove insoluble materials, and the amounts of N-carbamoylmethionine produced in the reaction mixtures were colorimetrically determined by measuring the absorbance at 420 nm. As a Control, the above procedures were repeated except that no hydantoin compound was employed.

1. customof an aqueous substrate solution prepared
2. customof the above cell suspension were prepared
3. customThe hydrolysis reaction
4. customImmediately after the completion of the reaction, 1.0 ml
5. customto remove insoluble materials

ORD record

ord-f119e163177a459f8efb89da3615bcce

Source dataset

uspto-grants-1980_12

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US04237227