反应详情

温度

20 °C

To a stirred solution of methyl 3-(4-(N-isobutyl-N-(2-methyl-5-(trifluoromethyl)phenyl)sulfamoyl)phenyl)propanoate (15 mg, 0.033 mmol) in tetrahydrofuran (THF) (1.5 mL) at room temperature, was added a solution of lithium hydroxide (approximately 0.785 mg, 0.033 mmol—tip of spatula used) in water (1.5 mL). The reaction mixture was stirred at 20° C. for 4 hours and then stood overnight. The mixture was concentrated in vacuo then partitioned between water (2 mL) and ethyl acetate (2×5 mL). The aqueous layer was separated (hydrophilic frit) and acidified, then extracted with ethyl acetate (5 mL). The organic layer was separated (hydrophobic frit) and concentrated in vacuo to give required product, 2.4 mg. LCMS (M+1) 444, RT 1.25 mins.

1. waitstood overnight
2. concentrationThe mixture was concentrated in vacuo
3. customthen partitioned between water (2 mL) and ethyl acetate (2×5 mL)
4. customThe aqueous layer was separated (hydrophilic frit)
5. extractionextracted with ethyl acetate (5 mL)
6. customThe organic layer was separated (hydrophobic frit)
7. concentrationconcentrated in vacuo