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HRID1765924

反应详情

EQUATION

反应方程式

HRID 1765924 的结构方程式

REACTANTS

反应物

4
结构：O=C[O-]
HCID283

Formic acid, ion(1-)

CHO2-

结构：[Na+].[OH-]
HCID14798

Sodium Hydroxide

HNaO

结构：NC(=O)C1=CN([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OC[C@H]3O[C@@H](n4cnc5c(N)ncnc54)[C@H](O)[C@@H]3O)[C@@H](O)[C@H]2O)C=CC1
HCID439153

Adenosine 5'-(trihydrogen diphosphate), P'→5'-ester with 1,4-dihydro-1-.beta.-D-ribofuranosyl-3-pyridinecarboxamide

C21H29N7O14P2

结构：O=C[O-].[Na+]
HCID2723810

Sodium formate

CHNaO2

PRODUCTS

生成物

1
结构：O=C(CO)COP(=O)(O)O
HCID668

2-Propanone, 1-hydroxy-3-(phosphonooxy)-

C3H7O6P

PROCEDURE

实验过程

For removing dX1P the acetaldehyde was evaporated and the solution was diluted with water to reach 30 ml. It was mixed with 3 ml 2.65 M sodium formate solution (8 mmol), and sodium hydroxide solution was added until a pH of 7.4 was reached. 23 U formate dehydrogenase (FDH), 6 mg NADH, 16 U RAMA and 20 U glycerolphosphate dehydrogenase (GDH) were added.

WORKUP

后处理

  1. customFor removing dX1P the acetaldehyde
  2. customwas evaporated
  3. additionthe solution was diluted with water
  4. additionwas added until a pH of 7.4