反应详情

To a solution of 4-(3-Chloro-phenyl)-2-(5,6-dimethoxy-benzoimidazol-1-yl)-thiazole-5-carboxylic acid (41 mg, 0.1 mmol) and thiophene-2-carboxylic acid hydrazide (14.2 mg, 0.1 mmol, Aldrich) in N,N-dimethylformamide (1 ml) was added HATU (38 mg, 0.1 mmol) and diisopropylethylamine (26 μl, 0.15 mmol). The mixture was stirred at ambient temperature overnight. The solvent was reduced in vacuo and water (3 ml) was added. After sonication, the crude was filtered and sent directly for purification with prep-HPLC to afford thiophene-2-carboxylic acid N′-[4-(3-chloro-phenyl)-2-(5,6-dimethoxy benzoimidazol-1-yl)-thiazole-5-carbonyl]-hydrazide (37.9 mg, 70.2%) as a white solid. MS m/z 540 (M+1).

1. additionwas added
2. customAfter sonication
3. filtrationthe crude was filtered
4. customsent directly for purification with prep-HPLC