Urea → HCID 178418489, compound

HRID 1786096Notes

Reaction equation

HRID 1786096 reaction equation
[O:1]=[CH:2][C@@H:3]([C@H:5]([C@@H:7]([C@@H:9](CO)[OH:10])[OH:8])[OH:6])[OH:4].NC(N)=O>>[CH2:2]([OH:1])[C@@H:3]([C@H:5]([C@@H:7]([CH2:9][OH:10])[OH:8])[OH:6])[OH:4]

Participants

3

Reactant 2

HCID 1176UreaHCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal

Product 1

HCID 178418489HCID 178418489, compound

Procedure

Approximately 2000 strains of osmophilic bacteria obtained as described above are cultured in individual microfermentors within a microfermentor array in a medium containing 20% (w/v) D-glucose, 0.1% urea, and 0.5% yeast extract at 30° C. for periods ranging from 12 hours to 5 days. The microfermentors have a working volume of 5 μl and are equipped with means to monitor biomass and oxygen concentration. Each microfermentor delivers oxygen to the interior of the microfermentor vessel via a PDMS aeration membrane. Each strain is introduced into 18 individual microfermentors using access channels. This allows 3 cultures to be terminated at each of 6 time points for each strain. The microfermentor array is maintained in a chamber as described in Example 3, which controls temperature and humidity. Biomass and dissolved oxygen concentration are monitored during the culture period, and data is accumulated using an appropriate software program. After an appropriate culture period (12, 24, 48, 72, 96, or 120 hours), all medium is removed from each microfermentor to be terminated at that time point and analyzed by HPLC to screen for a strain having the ability to produce xylitol.

Workup

  1. customApproximately 2000 strains of osmophilic bacteria obtained
  2. extractionextract at 30° C. for periods
  3. waitranging from 12 hours to 5 days
  4. customare equipped with means
  5. concentrationbiomass and oxygen concentration
  6. additionEach strain is introduced into 18 individual microfermentors
  7. customto be terminated at each of 6 time points for each strain
  8. temperatureThe microfermentor array is maintained in a chamber
  9. dissolutionBiomass and dissolved
  10. concentrationoxygen concentration
  11. customAfter an appropriate culture period (12, 24, 48, 72, 96, or 120 hours), all medium is removed from each microfermentor
  12. customto be terminated at that time point

Sources & evidence

ORD record
ord-9ad645f4c3a44b38ad4afc7d2f00546d
Source dataset
uspto-grants-2009_03
Patent number
US07507579B2

Reaction notes

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