反应详情

A mixture of 426 (70 mg, 0.15 mmol) and 6N NaOH (2 ml, 1.0 mmol) in a mixed solvents of EtOH/THF (2/1) (3 ml) was stirred at rt for 2 days. Neutralized with concentrated HCl to PH=6, treated with EtOAc (50 ml), the mixture was washed with H2O (2×20 ml) and brine (20 ml), dried with Na2SO4. Removal of the solvent gave 428A (55 mg, 93%) as a yellow solid. The product was used in next step without further purification. It has a retention time of 4.73 min (standard LC1 method, 8 min run). MS Found: (M+H)+=439.1

1. washthe mixture was washed with H2O (2×20 ml) and brine (20 ml)
2. dry with materialdried with Na2SO4
3. customRemoval of the solvent