Uracil → HCID 178418489, compound

Temperature

30 °C

The expression cassette PGtrpfs2-CoAraA constructed above was introduced into Candida parapsilosis in which URA3 was inactivated, which was smeared on the uracil-free solid selection medium (yeast nitrogen base 6.7 g/L, glucose 20 g/L, and agarose powder 15 g/L), followed by incubation for 2 days at 30° C. The colonies formed on the solid medium were the strains introduced with the cassette. The colonies were inoculated in 4 ml of YM medium (glucose 20 g/L, yeast extract 3 g/L, malt extract 3 g/L, and peptone 5 g/L), followed by shaking-culture at 150 rpm for 12 hours at 30° C. Then, the culture solution was smeared on 5-FOA solid medium (yeast nitrogen base 6.7 g/L, glucose 20 g/L, 5-FOA 0.8 g/L, uracil 0.1 g/L, and agarose powder 15 g/L), followed by incubation for 2 days at 30° C.

1. additionabove was introduced into Candida parapsilosis in which URA3
2. customThe colonies formed on the solid medium
3. additionintroduced with the cassette
4. extractionThe colonies were inoculated in 4 ml of YM medium (glucose 20 g/L, yeast extract 3 g/L, malt extract 3 g/L, and peptone 5 g/L)
5. waitfollowed by incubation for 2 days at 30° C.

ORD record

ord-c106cb2cf04244a8a22f452336890bc3

Source dataset

uspto-grants-2013_01

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US08343736B2