反应详情

[2′-(N′-Benzyl-N″-cyano-N-ethyl-guanidinomethyl)-6-methoxy-4′-trifluoromethyl-biphenyl-3-yl]-acetic acid ethyl ester (0.055 g, 0.1 mmol) in TIFF (2 mL) and H2O (0.5 mL) was treated with lithium hydroxide (0.02 g, 0.5 mmol), and the reaction was monitored by analytical LCMS. Once no starting material was seen, the mixture was diluted with EtOAc and H2O. Citric acid was added to neutralize the solution to pH 3, and the mixture was extracted with EtOAc. The combined organic layers were washed with H2O, dried over MgSO4, filtered, and concentrated twice from CH2Cl2 to give the title compound. M+H is 525.

1. extractionthe mixture was extracted with EtOAc
2. washThe combined organic layers were washed with H2O
3. dry with materialdried over MgSO4
4. filtrationfiltered
5. concentrationconcentrated twice from CH2Cl2