Tris(hydroxymethyl)aminomethane → Theophylline

HRID 1830491Notes

Reaction equation

HRID 1830491 reaction equation
[N:1]1([C:12](=[O:13])[C:11]2[NH:10][C:9](CCCC(O)=O)=[N:8][C:7]=2[N:5]([CH3:6])[C:3]1=[O:4])[CH3:2].CCN=C=NCCCN(C)C.Cl.C(O)C(N)(CO)CO>C1N(CCS(O)(=O)=O)CCOC1>[N:1]1([C:12](=[O:13])[C:11]2[NH:10][CH:9]=[N:8][C:7]=2[N:5]([CH3:6])[C:3]1=[O:4])[CH3:2]

Participants

5

Reactant 3

HCID 6503Tris(hydroxymethyl)aminomethaneHCID 950361H-Purine-8-butanoic acid, 2,3,6,7-tetrahydro-1,3-dimethyl-2,6-dioxo- (9CI)HCID 27239391,3-Propanediamine, N'-(ethylcarbonimidoyl)-N,N-dimethyl-, monohydrochloride

Product 1

HCID 2153Theophylline

Reagents, catalysts & solvents 1

HCID 781654-Morpholineethanesulfonic acidSolvent2×

Procedure

2 mg of theophylline-8-butanoic acid (Sigma) was dissolved in 4 ml of 0.1 M MES buffer solution. Thereafter, 7 mg of WSC hydrochloride and 5 mg of NHS were added thereto, and the mixture was stirred at room temperature for 15 minutes. Thereafter, 10 mg of BSA (Sigma) was dissolved in 2 ml of 0.1 M MES, and the obtained solution was added to the above reaction product. The obtained mixture was reacted at room temperature for 2 hours. 500 μl of a 1M Tris buffer solution (pH 7.5) was added to the mixture, and the reaction was terminated. Thereafter, dialysis was carried out against 0.1 M Tris buffer solution (pH 7.5).

Workup

  1. additionthe obtained solution was added to the above reaction product
  2. customThe obtained mixture was reacted at room temperature for 2 hours
  3. customthe reaction was terminated
  4. customThereafter, dialysis

Sources & evidence

ORD record
ord-9fe6855e099b4985b591845a4c85c8a3
Source dataset
uspto-grants-2006_06
Patent number
US07067270B2

Reaction notes

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