反应详情

To a mixture of 4-amino-1H-pyrazolo[3,4-d]pyrimidine-3-carbonitrile (76.6 g, 0.476 mol) and potassium carbonate (197 g, 1.427 mmol) in DMF (680 mL) at 0° C. was added tert-butyl chloroacetate (68.1 mL, 0.476 mol) dropwise while maintaining the internal temperature below 20° C. The resulting mixture was stirred for 22 h, filtered and washed with EtOAc (100 mL×2). The solid obtained was suspended in ice water (1.5 L), filtered, washed with ice water (500 mL) and ether (100 mL×2), then dried in vacuo to afford tert-butyl 2-(4-amino-3-cyano-1H-pyrazolo[3,4-d]pyrimidin-1-yl)acetate (81.6 g, 62.3% yield). MS (ES) m/z 275.0 (M+H+).

1. temperaturewhile maintaining the internal temperature below 20° C
2. filtrationfiltered
3. washwashed with EtOAc (100 mL×2)
4. customThe solid obtained
5. filtrationfiltered
6. washwashed with ice water (500 mL) and ether (100 mL×2)
7. customdried in vacuo