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HRID1856592

反应详情

EQUATION

反应方程式

HRID 1856592 的结构方程式

REACTANTS

反应物

4
结构：O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)CO
HCID107526

未命名化合物

结构：Cc1cn([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]2O)c(=O)[nH]c1=O
HCID451388

5-Methyluridine 5′-triphosphate

C10H17N2O15P3

结构：O=P([O-])([O-])OP(=O)([O-])[O-]
HCID644102

Diphosphate

O7P2-4

2 次

结构：O=P([O-])([O-])OP(=O)([O-])OP(=O)([O-])[O-]
HCID3440921

Triphosphate

O10P3-5

2 次

PRODUCTS

生成物

1
结构：Cc1cn([C@@H]2O[C@H](COP(=O)(O)OP(=O)(O)O)[C@@H](O)[C@H]2O)c(=O)[nH]c1=O
HCID23379797

未命名化合物

AUXILIARIES

试剂、催化剂与溶剂

2
结构：NC(CO)(CO)CO
溶剂HCID6503

Tromethamine

C4H11NO3

2 次

结构：O=S(=O)([O-])[O-].[NH4+].[NH4+]
溶剂HCID6097028

Ammonium sulfate

H8N2O4S

PROCEDURE

实验过程

5-Methyluridine 5'-triphosphate (0.0047 g, 0.009 mMol) was dissolved in 0.7 mL 1M Tris buffer (pH 8.5 containing 1M MgCl2) then 0.3 mL of a 0.25M glucose solution in 1M Tris buffer (pH 8.5 containing 1M MgCl2) added. A To timepoint was taken by reverse phase anion exchange HPLC. Hexokinase (EC 2.7.1.1, Boehringer Mannheim, from yeast overproducer), 75 units in 3.2M ammonium sulfate suspension, was added to the reaction which was incubated at 25° C. An HPLC was taken at 66 hours indicating complete conversion to diphosphate with no triphosphate remaining. Triphosphate retention: 17.5 minutes; diphosphate retention: 10.3 minutes.

WORKUP

后处理

  1. customwas incubated at 25° C
  2. custom17.5 minutes
  3. custom10.3 minutes