反应详情

A solution of 120.0 mg (0.45 mmol) 5-chloro-2-trichloromethyl-1H-benzoimidazole in 2 mL tetrahydrofuran (THF) was added slowly to a mixture of 53.5 μl (0.49 mmol) benzylamine and 373.8 mg (4.45 mmol, 10 equiv.) NaHCO3 in 8 mL THF and 4 mL water. The reaction mixture was stirred for 2 h at room temperature (20° C. to 25° C. in the following RT). The suspension was filtered and the filtrate was concentrated under reduced pressure. After purification by preparative RP-HPLC (CH3CN/H2O gradient and 0.05% formic acid) pure 5-Chloro-1H-benzoimidazole-2-carboxylic acid benzylamide was obtained as a white amorphous solid.

1. filtrationThe suspension was filtered
2. concentrationthe filtrate was concentrated under reduced pressure
3. customAfter purification by preparative RP-HPLC (CH3CN/H2O gradient and 0.05% formic acid) pure 5-Chloro-1H-benzoimidazole-2-carboxylic acid benzylamide
4. customwas obtained as a white amorphous solid