反应详情

Thiophosgene (10.0 μL, 0.13 mmol) was added to a stirred solution of 4d (0.11 mmol) in THF (900 μL) at −78° C. After approximately 45 min, the reaction mixture was poured into saturated aqueous sodium bicarbonate and extracted three times with methylene chloride. The combined organic extracts were washed with brine, dried (Na2SO4) and concentrated in vacuo. The crude residue was purified by preparative reversed phase HPLC on YMC Pack Pro C18 stationary phase (gradient elution; 0-75% acetonitrile/water as eluent, 0.1% TPA as modifier). Lyophilization of the purified fractions afforded the title compound 5a, m/z (ES) 506 (MH)+.

1. extractionextracted three times with methylene chloride
2. washThe combined organic extracts were washed with brine
3. dry with materialdried (Na2SO4)
4. concentrationconcentrated in vacuo
5. customThe crude residue was purified by preparative reversed phase HPLC on YMC Pack Pro C18 stationary phase (gradient elution; 0-75% acetonitrile/water as eluent, 0.1% TPA as modifier)