反应详情

Treat a solution of 4-(4,5-dimethyl-6-(4-(methylamino)piperidin-1-yl)pyridazin-3-yl)benzonitrile (102 mg, 0.32 mmol), 4-(trifluoromethyl)nicotinic acid (81 mg, 0.42 mmol), triethylamine (0.07 mL, 0.5 mmol) in CH2Cl2 (4 mL) with EDCI (99 mg, 0.52 mmol) and stir for 3 d. Pour the reaction mixture into H2O and extract with EtOAc. Wash the organic layer with H2O, dry over Na2SO4, filter, and concentrate under reduced pressure. Purify by flash silica gel chromatography (gradient of 0 to 10% MeOH in CH2Cl2). Dissolve the free base in MeOH (2 mL) and add 1 M HCl in Et2O (0.5 mL). Concentrate to provide the title compound (82 mg, 49%). ES/MS m/z 495.2 (M+1).

1. extractionextract with EtOAc
2. washWash the organic layer with H2O
3. dry with materialdry over Na2SO4
4. filtrationfilter
5. concentrationconcentrate under reduced pressure
6. customPurify by flash silica gel chromatography (gradient of 0 to 10% MeOH in CH2Cl2)
7. dissolutionDissolve the free base in MeOH (2 mL)
8. additionadd 1 M HCl in Et2O (0.5 mL)
9. concentrationConcentrate