反应详情

Next, 1.34 g (2.5 m moles) of H-Phe-Arg(NO2)-CHA was dissolved in 10 ml of 0.75N-NEM/DMF, to this solution was added 0.84 g (2.5 m moles) of BOC-D-Pro-SDP at 0° to 5° C. and reacted at room temperature for 18 hours. After the reaction was completed, the reaction mixture was diluted with 150 ml of AcOEt, and washed 4 times with 50 ml of cold 5% hydrochloric acid, and washed twice with 50 ml of an aqueous solution saturated with sodium chloride, then decolored and dried with activated carbon and anhydrous magnesium sulfate. After drying, magnesium sulfate and activated carbon were removed by filtration, and the solvent was removed from the filtrate by evaporation under reduced pressure to obtain crude product of BOC-D-Pro-Phe-Arg(NO2)-CHA, recrystallized from AcOEt to obtain 1.2 g (85.9%) of BOC-D-Pro-Phe-Arg(NO2)-CHA.

1. customreacted at room temperature for 18 hours
2. washwashed 4 times with 50 ml of cold 5% hydrochloric acid
3. washwashed twice with 50 ml of an aqueous solution saturated with sodium chloride
4. dry with materialdried with activated carbon and anhydrous magnesium sulfate
5. customAfter drying
6. custommagnesium sulfate and activated carbon were removed by filtration
7. customthe solvent was removed from the filtrate by evaporation under reduced pressure