反应详情

A solution of Fmoc-Xaa-OH (2 eq.), TBTU (2 eq.), HOBt (hydroxybenzotriazole) (2 eq.), DIEA (5.2 eq.) in NMP (1 ml/g resin) was added to the resin-bound free amine peptide and shaken for 60 min at room temperature and washed with NMP (5×).

1. washwashed with NMP (5×)