o-Dianisidine dihydrochloride → Hexose

HRID 1919226Notes

Reaction equation

HRID 1919226 reaction equation
[OH:1][C@@H:2]1[O:10][C@H:9]([CH2:11][OH:12])[C@@H:7]([OH:8])[C@H:5]([OH:6])[C@H:3]1[OH:4].COC1C=C(C2C=CC(N)=C(OC)C=2)C=CC=1N.Cl.Cl>P([O-])([O-])([O-])=O.[Na+].[Na+].[Na+]>[CH2:11]([OH:12])[CH:9]1[O:10][CH:2]([OH:1])[CH:3]([OH:4])[CH:5]([OH:6])[CH:7]1[OH:8]

Participants

4

Reactant 2

HCID 62311o-Dianisidine dihydrochlorideHCID 64689Beta-D-Glucose

Product 1

HCID 206Hexose

Reagents, catalysts & solvents 1

HCID 24243Trisodium PhosphateSolvent2×

Procedure

The assay was based on the method described by Sullivan and Ikawa (Biochimica et Biophysica Acta, 1973, 309:11-22), but modified to run in microtiter plates. An assay mixture contained 150 μl β-D-glucose (0.1 M in 0.1 M sodium phosphate buffer, pH 6.3), 120 μl 0.1 M sodium phosphate buffer, pH 6.3, 10 μl o-dianisidinedihydrochloride (Sigma D-3252, 3.0 mg/ml in H2O), 10 μl peroxidase (POD) (Sigma P-8125, 0.1 ml in 0.1 M sodium phosphate buffer, pH 6.3) and 10 μl enzyme (HOX) solution. Blanks were made by adding buffer in place of enzyme solution.

Workup

  1. additionby adding buffer in place of enzyme solution

Sources & evidence

ORD record
ord-e9fbd9602cac49b4b66086316853ab93
Source dataset
uspto-grants-2012_05
Patent number
USRE043341E1

Reaction notes

Loading…