反应详情

2-[4-({[2-[(tert-Butoxycarbonyl)amino]-5-(3-thienyl)phenyl]amino}-carbonyl)phenyl]-3-{[(methylamino)carbonyl]amino}propanoic acid (104 mg, 0.19 mmol), EDCI (54 mg, 0.28 mmol), and HOBT (34 mg, 0.25 mmol) were combined in DMF (3 mL) and stirred for 10 minutes before adding aniline (26 μL, 0.28 mmol). The reaction was then stirred at room temperature for 2 h. The mixture was directly purified by reverse phase HPLC (45-95% MeCN/water) to give the title compound as a white solid. MS (ESI) calcd [M+H]+ 614.2, found 614.1.

1. stirringThe reaction was then stirred at room temperature for 2 h
2. customThe mixture was directly purified by reverse phase HPLC (45-95% MeCN/water)