HRID1947270

反应详情

EQUATION

反应方程式

HRID 1947270 的结构方程式

AUXILIARIES

试剂、催化剂与溶剂

2

CONDITIONS

反应条件

温度
80 °C

PROCEDURE

实验过程

To a solution of methyl 4-[4-(cyclohexylamino)-8-(4-iodobenzyl)-2-oxo-1,3,8-triazaspiro[4.5]dec-3-en-1-yl]butanoate (Intermediate I.3.a.1, 150 mg, 0.29 mmol), zinc cyanide (24 mg, 0.20 mmol) in DMF (3 mL) was added tetrakis (33 mg, 0.03 mmol). The resulting solution was allowed to stir at 80° C. for 16 h. After 16 h, the reaction mixture was filtered, concentrated in vacuo and purified by reverse phase preparative HPLC (5- 95% MeCN/H2O containing 0.1% TFA, C18) to provide the desired product. LRMS (M+1)=466.6.

WORKUP

后处理

  1. waitAfter 16 h
  2. filtrationthe reaction mixture was filtered
  3. concentrationconcentrated in vacuo
  4. custompurified by reverse phase preparative HPLC (5- 95% MeCN/H2O containing 0.1% TFA, C18)