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HRID1967176

反应详情

EQUATION

反应方程式

HRID 1967176 的结构方程式

REACTANTS

反应物

4
结构：O=C(O)C(F)(F)F
HCID6422

未命名化合物

结构：CC(C)[C@H](NC(=O)OCC1c2ccccc2-c2ccccc21)C(=O)O
HCID688217

FMOC-L-valine

C20H21NO4

结构：CC(C)(C)OC(=O)NCCCC[C@H](NC(=O)OCC1c2ccccc2-c2ccccc21)C(=O)O
HCID2724628

Fmoc-Lys(Boc)-OH

C26H32N2O6

结构：CC(C)(C)OC(=O)NCCCC[C@H](N)C(=O)O
HCID2733283

未命名化合物

PRODUCTS

生成物

1
结构：CC(C)[C@H](NC(=O)[C@@H](N)CCCCN)C(=O)N[C@@H](CCCCN)C(=O)O
HCID448140

Lys-Val-Lys

C17H35N5O4

AUXILIARIES

试剂、催化剂与溶剂

1
结构：CCOCC
溶剂HCID3283

Diethyl Ether

C4H10O

PROCEDURE

实验过程

The protective peptide is built on 1.00 g (0.80 mmol) of H-Lys(Boc)-2-chlorotrityl resin using Fmoc-Val-OH, Fmoc-Lys(Boc)-OH and Palm-OSu. The resin is treated for 30 min with 8 ml of TFA 95% and the solution is instilled in 100 ml of Et2O. The precipitate is sucked off, washed and purified by prep. HPLC after drying. The substance is diluted in 30 ml of dioxan:water=4:6, treated overnight with 2.0 g of BioRad resin (acetate form), filtered, rotated and lyophilized.

WORKUP

后处理

  1. washwashed
  2. custompurified by prep
  3. customHPLC after drying
  4. additionThe substance is diluted in 30 ml of dioxan
  5. additionwater=4:6, treated overnight with 2.0 g of BioRad resin (acetate form)
  6. filtrationfiltered