Phosphoenolpyruvate → Succinic Acid

HRID 1970757ノート

反応式

[C:1]([OH:8])(=[O:7])[CH2:2][CH2:3][C:4]([OH:6])=[O:5].C=C(OP(O)(O)=O)C(O)=O.C(CC([O-])=O)(C(O)=O)=O.C([O-])(=O)C(CC([O-])=O)O.C([O-])(=O)C=O.[O:42]=[CH:43][C@@H:44]([C@H:46]([C@@H:48]([C@@H:50]([CH2:52][OH:53])[OH:51])[OH:49])[OH:47])[OH:45]>>[OH:53][CH2:52][C@@H:50]([C@H:48]([C@@H:46]([C@@H:44]([CH2:43][OH:42])[OH:45])[OH:47])[OH:49])[OH:51].[C:1]([OH:8])(=[O:7])[CH2:2][CH2:3][C:4]([OH:6])=[O:5]

参加物

反応物 6

HCID 1005Phosphoenolpyruvate

HCID 1110Succinic Acid4 回

HCID 107526(2R,3S,4R,5R)-2,3,4,5,6-pentahydroxyhexanal2 回

HCID 160434DL-Malate

HCID 3260017Oxaloacetate Ion

HCID 3614358Npc324351

生成物 2

HCID 1110Succinic Acid

HCID 5780Sorbitol

実験手順

Metabolic flux analysis was used to evaluate the effect of different carbon sources on succinic acid production in batch fermentations with A. succinogenes FZ45. The analyses established that the major pathway for succinic acid production in A. succinogenes FZ45 flows in the following manner: phosphoenolpyruvate (PEP→*oxaloacetate (OAA)→malate→fumarate→succinic acid. The glyoxylate shunt and the PEP-transport-system (PTS) appear not to be substantially used in the organism. Glucose fermentations reach a concentration of 61.7 g/L succinic acid with a yield of about 94% (succinic acid (g)/glucose (g)). Fermentations performed using a more reduced carbon source, such as sorbitol, produced higher amounts of succinic acid (77.3 g/L) with a higher yield (108% succinic acid (g)/glucose (g)), indicating that reducing power may become a limiting factor during the fermentation of glucose.

出典と根拠

ORDレコード: ord-da5f455381a947618b9d56ab9925c8b9
出典データセット: uspto-grants-2013_04
DOI: 10.6084/m9.figshare.5104873.v1
特許番号: US08431373B2

反応ノート

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