Hydrochloric Acid → HCID 46866168, compound

HRID 1970788Notes

Reaction equation

[NH2:1][C:2]1[C:7]([CH2:8][C:9]2[CH:14]=[CH:13][CH:12]=[CH:11][CH:10]=2)=[N:6][C:5]([C:15]2[CH:20]=[CH:19][C:18]([O:21][CH3:22])=[CH:17][CH:16]=2)=[CH:4][N:3]=1.C(N([CH2:28][CH3:29])CC)C.[CH2:30]([S:37](Cl)(=[O:39])=[O:38])[C:31]1[CH:36]=[CH:35][CH:34]=[CH:33][CH:32]=1.Cl>ClCCl>[CH2:8]([C:7]1[C:2]([N:1]([S:37]([CH2:30][C:29]2[CH:28]=[CH:33][CH:32]=[CH:31][CH:36]=2)(=[O:39])=[O:38])[S:37]([CH2:30][C:31]2[CH:36]=[CH:35][CH:34]=[CH:33][CH:32]=2)(=[O:39])=[O:38])=[N:3][CH:4]=[C:5]([C:15]2[CH:16]=[CH:17][C:18]([O:21][CH3:22])=[CH:19][CH:20]=2)[N:6]=1)[C:9]1[CH:10]=[CH:11][CH:12]=[CH:13][CH:14]=1

Participants

Conditions

Temperature: 0 °C

Procedure

2-Amino-3-benzyl-5-(4-methoxyphenyl)pyrazine (c-11) (prepared by the method described in Adamczyk M. et al., Org. Prep. Proced. Int., 33, 477-485 (2001)) (200 mg, 687 μmol) was dissolved in anhydrous dichloromethane (2 mL) and to this was added triethylamine (145 μL, 1.04 mmol), and cooled to 0° C. To this was added benzylsulfonyl chloride (196 mg, 1.03 mmol) and stirred for an hour after warming to room temperature. To this were sequentially added triethylamine (145 μL, 1.04 mmol), anhydrous dichloromethane (1.0 mL) and benzylsulfonyl chloride (131 mg, 687 μmol) and stirred for an hour. Furthermore, to this were sequentially added triethylamine (145 μL, 1.04 mmol), anhydrous dichloromethane (1.0 mL) and benzylsulfonyl chloride (131 mg, 687 μmol) and stirred for 2 h. To this was added 2 M hydrochloric acid to stop the reaction and, after separating the aqueous layer and organic layer, the organic layer was washed once with saturated brine and once with saturated aqueous solution of sodium sulfate, and then dried over anhydrous sodium sulfate. After removing anhydrous sodium sulfate by filtration, the filtrate was concentrated under reduced pressure with a rotary evaporater. The residue was purified by silica gel column chromatography (23 g, n-hexane/ethyl acetate=4/1) to give 3-benzyl-2-bis(benzylsulfonyl)amino-5-(4-methoxyphenyl)pyrazine (c-21) as a yellow amorphous (320 mg, 77.6%). Rf=0.63 (n-hexane/ethyl acetate=3/2). Mp. 78-80° C. UV (MeOH) λmax (log ε) 332.5 (4.31), 299.5 (4.20), 277.5 (4.06). UV (pH 7.4 PB) λmax (log ε) 342.5 (4.29), 303 (4.18), 280.5 (4.15). 1H NMR (400 MHz, DMSO-d6) δ 3.87 (s, 3H), 3.97 (s, 2H), 4.78 (s, 1H), 4.82 (s, 1H), 4.94 (s, 1H), 4.97 (s, 1H), 6.96-7.02 (AA′BB′, 2H), 7.17 (d, J=7.2 Hz, 2H), 7.19-7.33 (m, 3H), 7.34-7.44 (m, 10H), 7.92-7.98 (AA′BB′, 2H), 8.80 (s, 1H). 13C NMR (75.5 MHz, DMSO-d6) δ 37.4, 55.4, 61.0 (1C×2), 114.7 (2C), 126.4, 126.8 (1C×2), 128.1 (2C), 128.78 (2C), 128.84 (2C×2), 129.0, 129.3, 129.6 (2C), 131.6 (2C×2), 137.6, 138.5, 140.1, 151.9, 155.4, 161.5. IR (KBr, cm−1) 501, 611, 696, 773, 876, 922, 1028, 1144, 1163, 1250, 1354, 1373, 1422, 1431, 1454, 1495, 1514, 1607. HRMS (FAB+) m/z 600.1639 (M+H, C32H30N3O5S2 requires 600.1627).

Workup

temperatureafter warming to room temperature
stirringstirred for an hour
stirringstirred for 2 h
customthe reaction
customafter separating the aqueous layer and organic layer
washthe organic layer was washed once with saturated brine and once with saturated aqueous solution of sodium sulfate
dry with materialdried over anhydrous sodium sulfate
customAfter removing anhydrous sodium sulfate
filtrationby filtration
concentrationthe filtrate was concentrated under reduced pressure with a rotary evaporater
customThe residue was purified by silica gel column chromatography (23 g, n-hexane/ethyl acetate=4/1)

Sources & evidence

ORD record: ord-d0f1da07ab26400cb22ac78e632e870d
Source dataset: uspto-grants-2013_04
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US08431525B2

Reaction notes

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