反应详情

2-Chloro-1-(β-D-ribofuranosyl)-1H-benzimidazole (0.14 g) and 3-(4-benzyloxybutylamino)benzylamine (0.36 g) were suspended in isobutanol (5 mL). To the mixture was added triethylamine (0.56 mL), and the mixture was refluxed for 16 hours. The reaction mixture was concentrated under reduced pressure, and the obtained residue was purified by preparative reverse phase column chromatography (Shiseido CAPSELL PAC C18UG80, 5 μm, 20×50 mm, flow rate 30 mL/minutes linear gradient, water/methanol=70/30-10/90) to give the title compound (0.08 g).

1. temperaturethe mixture was refluxed for 16 hours
2. concentrationThe reaction mixture was concentrated under reduced pressure
3. customthe obtained residue was purified by preparative reverse phase column chromatography (Shiseido CAPSELL PAC C18UG80, 5 μm, 20×50 mm, flow rate 30 mL/minutes linear gradient, water/methanol=70/30-10/90)