Creatine → Nicotinamide

HRID 2040737Notes

Reaction equation

P(O)(O)(O)=O.O=[C:7]([CH2:9][N:10]([C:12](=N)N)C)O.O=C(CN(C(=N)N)C)O.[Mg+2].[Cl-].[Cl-].P(OC[C@H]1[O:45][C@@H:44]([N:46]2C3N=CN=C(N)C=3N=C2)[C@H:43](O)[C@@H:42]1O)(OP(OP(O)(O)=O)(O)=O)(=O)O>O>[C:44]([NH2:46])(=[O:45])[C:43]1[CH:42]=[CH:7][CH:9]=[N:10][CH:12]=1

Participants

Conditions

Temperature: 80 °C

Procedure

Creatine phosphate (14.5 mg) and creatine kinase (0.1 mg) were dissolved in a mixture of TRIS buffer (pH 7.5, 15 mM MgCl2, 960 μl) and ATP (100 mM/l in H2O, 40 μl). A solution of the riboside (compound 14, or analogue thereof, as given above) (100 mg/ml in H2O, 100 μl) was added, followed by the addition of ribosyl kinase (0.7 U/ml, 230 μl). The reaction mixture was incubated for 16 hrs. at 37° C. Next, the reaction mixture was warmed up to 80° C., filtered and analyzed by high performance liquid chromatography (“HPLC”).

Workup

additionwas added
additionfollowed by the addition of ribosyl kinase (0.7 U/ml, 230 μl)
customat 37° C
filtrationfiltered

Sources & evidence

ORD record: ord-a56d13ae40ce41dfaf6a1267e17586a8
Source dataset: uspto-grants-2013_11
DOI: 10.6084/m9.figshare.5104873.v1
Patent number: US08580949B2

Reaction notes

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