Magnesium Sulfate Heptahydrate → Cetalox

HRID 2066363Notes

Reaction equation

HRID 2066363 reaction equation
O.O.O.O.O.O.O.S([O-])([O-])(=O)=O.[Mg+2].[CH3:14][C@@:15]12[C@@H:24]([CH2:25][CH2:26][C@@](O)(C=C)C)[C@@:23]([OH:33])([CH3:32])[CH2:22][CH2:21][C@H:20]1[C:19]([CH3:35])([CH3:34])[CH2:18][CH2:17][CH2:16]2>[Pt]>[CH3:32][C:23]12[CH2:22][CH2:21][CH:20]3[C:15]([CH3:14])([CH2:16][CH2:17][CH2:18][C:19]3([CH3:34])[CH3:35])[CH:24]1[CH2:25][CH2:26][O:33]2

Participants

4

Reactant 2

HCID 24843Magnesium Sulfate HeptahydrateHCID 163263Sclareol

Product 1

HCID 107166Cetalox

Reagents, catalysts & solvents 1

HCID 23939PlatinumCatalyst

Procedure

In this example, the ability of Ascomycete sp. KSM-JL2842 for producing a dodecahydro-3a,6,6,9a-tetramethylnaphtho[2,1-b]furan intermediate was examined. At the outset, a platinum loopful of Ascomycete sp. KSM-JL2842 was inoculated into 2.1% YM broth (Becton Dickinson), shake-culture was conducted at 25° C. for 2 days, and the resultants were designated as starter strains. Subsequently, the starter strains were inoculated into a medium containing 2.1% YM broth, 0.1% magnesium sulfate heptahydrate, 1% Tween 80, and 2% sclareol to a concentration of 2%, and shake culture was conducted at 25° C. The culture solution was subjected to extraction and GC analysis via the method of Example 1, and the amounts of the dodecahydro-3a,6,6,9a-tetramethylnaphtho[2,1-b]furan intermediates to be produced were determined. The results are shown in Table 3. The units for the numerical values shown in Table 3 are “g/l.”

Workup

  1. customwas conducted at 25° C
  2. extractionThe culture solution was subjected to extraction and GC analysis via the method of Example 1

Sources & evidence

ORD record
ord-291a1d7ddaef492d9c0eb23b0436c2f6
Source dataset
uspto-grants-2012_10
Patent number
US08293518B2

Reaction notes

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