反应详情

To a methylene chloride (10 ml) solution of the (3,6-dichloropyridin-2-yl)(pyridin-4-yl)methanol (161 mg, 0.631 mmol) obtained in Referential Example 25 were added triethylamine (208 μl, 1.89 mmol) and thionyl chloride (138 μl, 1.89 mmol). The resulting mixture was stirred at room temperature for 4 hours, followed by concentration under reduced pressure. To the residue thus obtained was added ethyl acetate. The resulting mixture was washed sequentially with a saturated aqueous solution of sodium bicarbonate and brine, dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure. The residue thus obtained was dissolved in acetonitrile (10 ml). To the resulting solution were added 4-chlorobenzenethiol (137 mg, 0.947 mmol) and potassium carbonate (131 mg, 0.947 mmol). Under a nitrogen atmosphere, the resulting mixture was stirred at room temperature for 2 days and then, stirred at 60° C. for 4 hours. After cooling to room temperature, the reaction mixture was concentrated under reduced pressure. To the residue was added ethyl acetate. The resulting mixture was washed sequentially with water and brine, dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure. The residue thus obtained was subjected to flash chromatography. The fraction obtained from the 40% ethyl acetate/hexane eluate was concentrated under reduced pressure. The residue thus obtained was dissolved in methanol (10 ml). To the resulting solution were added 30% aqueous hydrogen peroxide (3 ml) and hexaammonium heptamolybdate tetrahydrate (73 mg). After the resulting mixture was stirred at room temperature for 5 hours, methanol was distilled off under reduced pressure. To the solution thus obtained was added a saturated aqueous solution of sodium bicarbonate, followed by extraction with methylene chloride. The organic layer was dried over anhydrous sodium sulfate and filtered. The filtrate was concentrated under reduced pressure. The residue thus obtained was subjected to flash chromatography. The fraction obtained from the methanol:methylene chloride=1:80 eluate was concentrated under reduced pressure to give the title compound (49 mg, 0.118 mmol, 19%) as a white solid.

1. concentrationfollowed by concentration under reduced pressure
2. customTo the residue thus obtained
3. washThe resulting mixture was washed sequentially with a saturated aqueous solution of sodium bicarbonate and brine
4. dry with materialdried over anhydrous sodium sulfate
5. filtrationfiltered
6. concentrationThe filtrate was concentrated under reduced pressure
7. customThe residue thus obtained
8. stirringUnder a nitrogen atmosphere, the resulting mixture was stirred at room temperature for 2 days
9. stirringstirred at 60° C. for 4 hours
10. temperatureAfter cooling to room temperature
11. concentrationthe reaction mixture was concentrated under reduced pressure
12. additionTo the residue was added ethyl acetate
13. washThe resulting mixture was washed sequentially with water and brine
14. dry with materialdried over anhydrous sodium sulfate
15. filtrationfiltered
16. concentrationThe filtrate was concentrated under reduced pressure
17. customThe residue thus obtained
18. concentrationwas concentrated under reduced pressure
19. customThe residue thus obtained
20. stirringAfter the resulting mixture was stirred at room temperature for 5 hours
21. distillationmethanol was distilled off under reduced pressure
22. customTo the solution thus obtained
23. extractionfollowed by extraction with methylene chloride
24. dry with materialThe organic layer was dried over anhydrous sodium sulfate
25. filtrationfiltered
26. concentrationThe filtrate was concentrated under reduced pressure
27. customThe residue thus obtained
28. concentrationmethylene chloride=1:80 eluate was concentrated under reduced pressure