反应详情

A solution of 44C (30 mg, 0.05 mmol) in CH2Cl2 (1 mL) at 0° C. was treated with TFA (0.05 mL). The solution was warmed to room temperature and stirred for 16 hours. The crude mixture was purified by chromatography (SiO2, 50% EtOAc in hexane) to afford 44D (15 mg, 68%). HPLC Retention Time=3.09 min (YMC ODSA 5 u C18 4.6×50 mm, 10-90% aqueous methanol containing 0.1% TFA, 4 min gradient, monitored at 220 nm) [M+H+]=439.

1. customThe crude mixture was purified by chromatography (SiO2, 50% EtOAc in hexane)