反应详情

To a solution of 4-[3-(1H-tetrazol-5-yl)-phenoxazin-10-yl]-piperidine-1-carboxylic acid tert-butyl ester, 5a (0.265 g, 0.61 mmol) in DMF (2 mL) was added a 4N hydrochloric acid solution (4 mL). The mixture was stirred for 2 h at rt. Another 4 mL of the 4N HCl solution was added, and the mixture was stirred for 16 h at rt. The solvent was removed via evaporation, and the residue was purified via reverse phase chromatography (eluent gradient: 10 to 40% acetonitrile in water containing 0.1% TFA) to yield 0.12 g (44%) of 10-piperidin-4-yl-3-(1H-tetrazol-5-yl)-10H-phenoxazine, 6a as a TFA salt. MS m/z (MH+) 335.0.

1. stirringthe mixture was stirred for 16 h at rt
2. customThe solvent was removed via evaporation
3. customthe residue was purified via reverse phase chromatography (eluent gradient: 10 to 40% acetonitrile in water containing 0.1% TFA)