Methyl iodide → HCID 58346719, compound

To 4 mL DMF cooled to 0° C. was added sodium hydride (16 mg (60%), 0.40 mmol). To the slightly yellow and heterogeneous mixture was added 2-[6-({3-chloro-4-[(3-fluorobenzyl)oxy]phenyl}amino)-4,5-dihydro-2H-pyrimido[5′,4′:4,5]thieno[2,3-e]indazol-2-yl]ethanol (100 mg, 0.19 mmol) as a 1 mL DMF solution, upon which the contents become brown and homogenous. The contents were allowed to stir with warming to it over 10 min., and added was iodomethane (0.01 mL, 0.23 mmol). The mixture was stirred for 90 min., after which time water (0.5 mL) was added. The contents were concentrated under reduced pressure to dryness, and the residue diluted with CH2Cl2 (10 mL). The organic layer was washed with water (3×10 mL). The organic layer was dried over MgSO4, filtered, and the solvent removed under reduced pressure. The crude residue was purified by reverse phase HPLC. The fractions for each respective product were combined and diluted with CH2Cl2 (50 mL), and neutralized with NaHCO3 (50 mL). The respective organic layers were dried over MgSO4, filtered, and the solvent removed under reduced pressure to afford the final pure products. Example 312 (15 mg, 15%) and N-{3-chloro-4-[(3-fluorobenzyl)oxy]phenyl}-2-(2-methoxyethyl)-N-methyl-4,5-dihydro-2H-pyrimido[5′,4′:4,5]thieno[2,3-e]indazol-6-amine (8 mg, 8%) were each collected as white solids. Data for example 312: 1H-NMR (CD2Cl2-d2) δ 8.50 (s, 1H), 7.39 (s, 1H), 7.20-7.28 (m, 1H), 7.07-7.17 (m, 2H), 6.90-6.99 (m, 2H), 6.72 (d, 1H), 6.62 (d, 1H), 4.99 (s, 2H), 4.08 (t, 2H), 3.80 (t, 2H), 3.42 (s, 3H), 2.48 (t, 2H), 2.20 (t, 2H); LCMS RT=3.32 min; [M+H]+=536.3. Data for N-{3-chloro-4-[(3-fluorobenzyl)oxy]phenyl}-2-(2-methoxyethyl)-N-methyl-4,5-dihydro-2H-pyrimido[5′,4′:4,5]thieno[2,3-e]indazol-6-amine: 1H-NMR (CD2Cl2-d2) δ 8.52 (s, 1H), 7.40 (s, 1H), 7.20-7.29 (m, 1H), 7.07-7.17 (m, 2H), 7.00 (s, 1H), 6.90-6.99 (m, 1H), 6.70 (d, 1H), 6.60 (d, 1H), 4.99 (s, 2H), 4.10 (t, 2H), 3.60 (t, 2H), 3.42 (s, 3H), 3.02 (s, 3H), 2.48 (t, 2H), 2.21 (t, 2H); LCMS RT=3.67 min; [M+H]+=550.4.

1. temperaturewith warming to it over 10 min.
2. additionadded
3. stirringThe mixture was stirred for 90 min.
4. concentrationThe contents were concentrated under reduced pressure to dryness
5. additionthe residue diluted with CH2Cl2 (10 mL)
6. washThe organic layer was washed with water (3×10 mL)
7. dry with materialThe organic layer was dried over MgSO4
8. filtrationfiltered
9. customthe solvent removed under reduced pressure
10. customThe crude residue was purified by reverse phase HPLC
11. additiondiluted with CH2Cl2 (50 mL)
12. dry with materialThe respective organic layers were dried over MgSO4
13. filtrationfiltered
14. customthe solvent removed under reduced pressure
15. customto afford the final pure products
16. customeach collected as white solids

ORD record

ord-937176a9c0fe4c12a2e1c1402b6520f8

Source dataset

uspto-grants-2012_06

DOI

10.6084/m9.figshare.5104873.v1

Patent number

US08207172B2