1-Ethyl-3-(3'-dimethylaminopropyl)carbodiimide → 1-[(R)-3-(2-{4-[1-(2-Ethoxy-ethyl)-1H-benzoimidazol-2-yl]-[1,4]diazepan-1-yl}-ethyl)-3-phenyl-pyrrolidin-1-yl]-1-[2-methoxy-5-(3-methylsulfanyl-[1,2,4]triazol-4-yl)-phenyl]-methanone

HRID 2230745Notes

Reaction equation

HRID 2230745 reaction equation
[CH3:1][O:2][C:3]1[CH:11]=[CH:10][C:9]([N:12]2[CH:16]=[N:15][N:14]=[C:13]2[S:17][CH3:18])=[CH:8][C:4]=1[C:5]([OH:7])=O.Cl.Cl.[CH2:21]([O:23][CH2:24][CH2:25][N:26]1[C:30]2[CH:31]=[CH:32][CH:33]=[CH:34][C:29]=2[N:28]=[C:27]1[N:35]1[CH2:41][CH2:40][CH2:39][N:38]([CH2:42][CH2:43][C@:44]2([C:49]3[CH:54]=[CH:53][CH:52]=[CH:51][CH:50]=3)[CH2:48][CH2:47][NH:46][CH2:45]2)[CH2:37][CH2:36]1)[CH3:22].O.ON1C2C=CC=CC=2N=N1.CN(C)CCCN=C=NCC.C(N(C(C)C)CC)(C)C>ClCCl>[CH2:21]([O:23][CH2:24][CH2:25][N:26]1[C:30]2[CH:31]=[CH:32][CH:33]=[CH:34][C:29]=2[N:28]=[C:27]1[N:35]1[CH2:41][CH2:40][CH2:39][N:38]([CH2:42][CH2:43][C@:44]2([C:49]3[CH:54]=[CH:53][CH:52]=[CH:51][CH:50]=3)[CH2:48][CH2:47][N:46]([C:5]([C:4]3[CH:8]=[C:9]([N:12]4[CH:16]=[N:15][N:14]=[C:13]4[S:17][CH3:18])[CH:10]=[CH:11][C:3]=3[O:2][CH3:1])=[O:7])[CH2:45]2)[CH2:37][CH2:36]1)[CH3:22]

Participants

7

Reactant 5

HCID 159081-Ethyl-3-(3'-dimethylaminopropyl)carbodiimideHCID 81531DiisopropylethylamineHCID 27960291-Hydroxybenzotriazole hydrateHCID 184647022-Methoxy-5-(3-methylsulfanyl-[1,2,4]triazol-4-yl)-benzoic acidHCID 701481071-(2-ethoxy-ethyl)-2-{4-[2-((S)-3-phenyl-pyrrolidin-3-yl)-ethyl]-[1,4]diazepan-1-yl}-1H-benzoimidazole dihydrochloride

Product 1

HCID 599022681-[(R)-3-(2-{4-[1-(2-Ethoxy-ethyl)-1H-benzoimidazol-2-yl]-[1,4]diazepan-1-yl}-ethyl)-3-phenyl-pyrrolidin-1-yl]-1-[2-methoxy-5-(3-methylsulfanyl-[1,2,4]triazol-4-yl)-phenyl]-methanoneYield 17.1%

Reagents, catalysts & solvents 1

HCID 6344DichloromethaneSolvent

Procedure

To a mixture of 2-methoxy-5-(3-methylsulfanyl-[1,2,4]triazol-4-yl)-benzoic acid (0.27 g, 0.42 mmol), 1-(2-ethoxy-ethyl)-2-{4-[2-((S)-3-phenyl-pyrrolidin-3-yl)-ethyl]-[1,4]diazepan-1-yl}-1H-benzoimidazole dihydrochloride (0.23 g, 0.42 mmol), 1-hydroxybenzotriazole hydrate (0.068 g, 0.51 mmol), and 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide (0.97 g, 0.51 mmol), add via syringe under argon anhydrous dichloromethane (15 mL). Add diisopropylethylamine (0.22 mL, 1.26 mmol) and stir at room temperature for 20 hours. Dilute with dichloromethane and wash twice with water. Wash with saturated NaHCO3 solution, dry (MgSO4) the organic phase, filter and concentrate to an oil. Purify the oil by flash chromatography (silica gel, 3.5×15.2 cm) eluting with dichloromethane and then with 10% methanol in dichloromethane. Concentrate the appropriate fractions to afford 0.051 g of the title compound, Rf=0.26 (silica gel, 10% methanol in dichloromethane). The compound may be converted to the dihydrochloride salt by methods well known in the art.

Workup

  1. additionadd via syringe under argon anhydrous dichloromethane (15 mL)
  2. washwash twice with water
  3. washWash with saturated NaHCO3 solution
  4. dry with materialdry (MgSO4) the organic phase
  5. filtrationfilter
  6. concentrationconcentrate to an oil
  7. customPurify the oil by flash chromatography (silica gel, 3.5×15.2 cm)
  8. washeluting with dichloromethane
  9. concentrationConcentrate the appropriate fractions

Sources & evidence

ORD record
ord-25f6f960a2814cbebafb0ced7bc99a60
Source dataset
uspto-grants-2002_07
Patent number
US06423704B1

Reaction notes

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