反应详情

7.2 gm of 2-(2-methoxy-4-methylsulfinyl-phenyl)-1H-imidazo[4,5-b]pyridine were dissolved in a mixture of a phosphate buffer of pH 7.2 and 120 ml of 3% hydrogen peroxide, and a solution of 9.3 gm of ethylenediamine tetraacetic acid, 4.4 gm of ascorbic acid, 3.8 gm of iron(II) sulfate in a phosphate buffer of pH 7.2 was added. After 30 minutes the reaction mixture was evaporated in vacuo, and the residue was purified by chromatography on a polystyrene resin column (XAD-2 of Rohm & Haas) with water and methanol as eluants. After evaporation of the eluate the two compounds were separated by means of chromatography on silicagel [eluant: chloroform/methanol/ammonia water=85/15/2 (v/v)]. 2-(2-Methoxy-4-methylsulfinyl-phenyl)-6-hydroxy-1H-imidazo[4,5-b]pyridine was obtained as yellow powder.

1. customAfter 30 minutes the reaction mixture was evaporated in vacuo
2. customthe residue was purified by chromatography on a polystyrene resin column (XAD-2 of Rohm & Haas) with water and methanol as eluants
3. customAfter evaporation of the eluate the two compounds
4. customwere separated by means of chromatography on silicagel [eluant: chloroform/methanol/ammonia water=85/15/2 (v/v)]