Triethylamine → HCID 86618156, compound

HRID 2246595Notes

Reaction equation

HRID 2246595 reaction equation
[NH2:1][C@H:2]1[CH2:6][CH2:5][N:4]([C@H:7]2[CH2:12][CH2:11][C@@H:10]([NH:13][C:14](=[O:20])[O:15][C:16]([CH3:19])([CH3:18])[CH3:17])[CH2:9][C@H:8]2[NH:21][C:22](=[O:25])[CH2:23][CH3:24])[C:3]1=[O:26].Cl[C:28]1[C:37]2[C:32](=[CH:33][CH:34]=[C:35]([C:38]([F:41])([F:40])[F:39])[CH:36]=2)[N:31]=[CH:30][N:29]=1.C(N(CC)CC)C>C(O)(C)C>[O:26]=[C:3]1[C@@H:2]([NH:1][C:28]2[C:37]3[C:32](=[CH:33][CH:34]=[C:35]([C:38]([F:40])([F:41])[F:39])[CH:36]=3)[N:31]=[CH:30][N:29]=2)[CH2:6][CH2:5][N:4]1[C@H:7]1[CH2:12][CH2:11][C@@H:10]([NH:13][C:14](=[O:20])[O:15][C:16]([CH3:17])([CH3:19])[CH3:18])[CH2:9][C@H:8]1[NH:21][C:22](=[O:25])[CH2:23][CH3:24]

Participants

5

Reactant 3

HCID 8471TriethylamineHCID 210750954-Chloro-6-(trifluoromethyl)quinazolineHCID 86618157HCID 86618157, compound

Product 1

HCID 86618156HCID 86618156, compound

Reagents, catalysts & solvents 1

HCID 3776IsopropanolSolvent

Conditions

Temperature
50 °C

Procedure

To a solution of tert-butyl (1R,3R,4S)-4-((S)-3-amino-2-oxopyrrolidin-1-yl)-3-propionamidocyclohexylcarbamate (650 mg, 1.764 mmol) in 5 mL of isopropanol was added 4-chloro-6-(trifluoromethyl)quinazoline (492 mg, 2.117 mmol) and triethylamine (0.492 mL, 3.53 mmol). The mixture was stirred at 50° C. for 4 h. The solvent was removed under reduced pressure and the residue was diluted with 30 mL of 20% AcOH in water before being extracted with diethyl ether (2×20 mL). The ethereal layer was extracted with 20% AcOH solution in water (3×30 mL). The aqueous layers were combined and basified with Na2CO3 and extracted with methylene chloride (3×50 mL). The organic extracts were combined, washed with brine (40 mL), dried over anhydrous Na2SO4 and concentrated under reduced pressure to give tert-butyl (1R,3R,4S)-4-((S)-2-oxo-3-(6-(trifluoromethyl)quinazolin-4-ylamino)pyrrolidin-1-yl)-3-propionamidocyclohexylcarbamate, LC/MS found [M+H]+=565. The entirety of this crude product was dissolved in 5 mL of methylene chloride. The resultant solution was charged with TFA (3 mL), stirred at room temperature for 30 min, and then concentrated in vacuo. The residue was purified with preparative reverse-phase HPLC to give the title compound as its TFA salt in aqueous methanol solution. This solution was concentrated in vacuo to remove the methanol and the residue solution was basified with saturated NaHCO3 and extracted with methylene chloride (3×50 mL), dried (Na2SO4) and concentrated in vacuo to give the title compound as its free base (550 mg, 1.184 mmol, 67% yield). LC/MS found [M+H]+=465. 1H-NMR (400 MHz, CD3OD), δ ppm: 8.77 (1H, s), 8.57 (1H, s), 8.03 (1H, dd, J=9.16, 2.03 Hz), 7.88 (1H, d, J=8.65 Hz), 5.24 (1H, t, J=8.65 Hz), 4.55 (1H, d, J=3.56 Hz), 3.97-4.05 (1H, m), 3.52-3.67 (2H, m), 3.35-3.41 (1H, m), 2.47-2.58 (1H, m), 2.20-2.34 (3H, m), 1.62-2.04 (6H, m), 1.14 (3H, t, J=7.63 Hz).

Workup

  1. customThe solvent was removed under reduced pressure
  2. additionthe residue was diluted with 30 mL of 20% AcOH in water
  3. extractionbefore being extracted with diethyl ether (2×20 mL)
  4. extractionThe ethereal layer was extracted with 20% AcOH solution in water (3×30 mL)
  5. extractionextracted with methylene chloride (3×50 mL)
  6. washwashed with brine (40 mL)
  7. dry with materialdried over anhydrous Na2SO4
  8. concentrationconcentrated under reduced pressure

Sources & evidence

ORD record
ord-3fab68a15c42416fb78d956edbb8778f
Source dataset
uspto-grants-2010_03
Patent number
US07687508B2

Reaction notes

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