Ethylenediaminetetraacetic Acid → HCID 88428908, compound

HRID 2254523Notes

Reaction equation

HRID 2254523 reaction equation
Cl.[N+:2]([C@@:5]([C:21]([OH:23])=[O:22])([CH2:16][CH2:17][CH2:18][CH2:19][NH2:20])[N:6]([N+:13]([O-:15])=[O:14])[C:7]1[CH:12]=[CH:11][CH:10]=[CH:9][CH:8]=1)([O-:4])=[O:3].C(N(CC(O)=O)CC(O)=O)CN(CC(O)=O)CC(O)=[O:29].C[N:45]([CH3:48])[CH:46]=[O:47]>P([O-])([O-])([O-])=O.[Na+].[Na+].[Na+]>[C:46]1(=[O:47])[NH:45][C:48](=[O:29])[CH:16]=[CH:5]1.[N+:2]([C@@:5]([C:21]([OH:23])=[O:22])([CH2:16][CH2:17][CH2:18][CH2:19][NH2:20])[N:6]([N+:13]([O-:15])=[O:14])[C:7]1[CH:8]=[CH:9][CH:10]=[CH:11][CH:12]=1)([O-:4])=[O:3]

Participants

5

Reactant 3

HCID 6049Ethylenediaminetetraacetic AcidHCID 6228DimethylformamideHCID 88430126dinitrophenyl-L-lysine hydrochloride

Product 1

HCID 88428908HCID 88428908, compound

Reagents, catalysts & solvents 1

HCID 24243Trisodium PhosphateSolvent

Procedure

An aliquot (1.5 ml) of 5.5 mM dinitrophenyl-L-lysine hydrochloride in 0.1M sodium phosphate buffer, pH 7.0, containing 5 mM EDTA was incubated with 0.15 ml of 5.5 mM N-succinimidyl-6-maleimidohexanoate in N,N-dimethylformamide at 30° C. for 30 minutes.

Sources & evidence

ORD record
ord-5dcd1c8602824f1086aef58f8e275ce8
Source dataset
uspto-grants-1993_08
Patent number
US05236849

Reaction notes

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