Ethylenediaminetetraacetic Acid → HCID 88428908, compound

HRID 2254524Notes

Reaction equation

HRID 2254524 reaction equation
[N+:1]([C@@:4]([C:20]([OH:22])=[O:21])([CH2:15][CH2:16][CH2:17][CH2:18][NH2:19])[N:5]([N+:12]([O-:14])=[O:13])[C:6]1[CH:11]=[CH:10][CH:9]=[CH:8][CH:7]=1)([O-:3])=[O:2].C(N(CC(O)=O)CC(O)=O)CN(CC(O)=O)CC(O)=[O:28].C[N:44]([CH3:47])[CH:45]=[O:46]>P([O-])([O-])([O-])=O.[Na+].[Na+].[Na+]>[C:45]1(=[O:46])[NH:44][C:47](=[O:28])[CH:15]=[CH:4]1.[N+:1]([C@@:4]([C:20]([OH:22])=[O:21])([CH2:15][CH2:16][CH2:17][CH2:18][NH2:19])[N:5]([N+:12]([O-:14])=[O:13])[C:6]1[CH:7]=[CH:8][CH:9]=[CH:10][CH:11]=1)([O-:3])=[O:2]

Participants

5

Reactant 3

HCID 6049Ethylenediaminetetraacetic AcidHCID 6228DimethylformamideHCID 54195575HCID 54195575, compound

Product 1

HCID 88428908HCID 88428908, compound

Reagents, catalysts & solvents 1

HCID 24243Trisodium PhosphateSolvent

Procedure

An aliquot (1.5 ml) of 8.8 mM dinitrophenyl-L-lysine (Tokyo Kasei, Tokyo, Japan) in 0.1M sodium phosphate buffer, pH 7.0, containing 5 mM EDTA was incubated with 0.15 ml of 8.8 mM N-succinimidyl-6-maleimidohexanoate (Dojin Kagaku, Kumamoto, Japan) in N,N-dimethylformamide at 30° C. for 30 minutes.

Sources & evidence

ORD record
ord-8156cfeeb5774ca6a5b1bae79b13068d
Source dataset
uspto-grants-1993_08
Patent number
US05236849

Reaction notes

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