反应详情

温度

20 °C

H-Glu(OtBu)-Thr(tBu)-OtBu (1.27 g, 3.05 mmol) and Z-Gly-Gly-Gly-OH (0.99 g, 3.05 mmol) were dissolved in DMF (20 ml). To the solution were added HOBT (453 mg, 3.35 mmol) and WSC (643 mg, 3.35 mmol). The mixture was stirred for 8 hours at 20° C. The reaction mixture was concentrated, which was suspended in 0.2M aqueous solution of citric acid (70 ml), followed by extraction with ethyl acetate (100 ml, 70 ml). The ethyl acetate layers were combined and washed with a 10% (w/v) aqueous solution of ammonium chloride, 5% (w/v) aqueous solution of sodium hydrogencarbonate, water and a saturated aqueous saline solution, successively, which was dried over anhydrous sodium sulfate, followed by concentration. To the concentrate was added ether-hexane. Resulting precipitates were collected by filtration to afford Z-Gly-Gly-Gly-Glu(OtBu)-Thr(tBu)-OtBu (Z-P-2) as a while powdery product (1.87 g, 85%).

1. concentrationThe reaction mixture was concentrated
2. extractionfollowed by extraction with ethyl acetate (100 ml, 70 ml)
3. washwashed with a 10% (w/v) aqueous solution of ammonium chloride, 5% (w/v) aqueous solution of sodium hydrogencarbonate, water
4. dry with materiala saturated aqueous saline solution, successively, which was dried over anhydrous sodium sulfate
5. concentrationfollowed by concentration
6. additionTo the concentrate was added ether-hexane
7. customResulting
8. customprecipitates
9. filtrationwere collected by filtration