Ethylenediaminetetraacetic Acid → Imidazole-4-acetic acid
Reaction equation
C(O)[C:2](N)([CH2:5][OH:6])CO.Cl.C(S)[C@@H](O)[C@H]([OH:15])CS.[CH2:18]([N:29]([CH2:34]C(O)=O)CC(O)=O)[CH2:19][N:20](CC(O)=O)CC(O)=O>>[CH:19]1[N:20]=[CH:34][NH:29][C:18]=1[CH2:2][C:5]([OH:6])=[O:15]Participants
4
Procedure
The cell pellet was homogenized in 6 volumes 0.1 M Tris-HCl pH 8, 0.2 M NaCl, 5 mM DTT, 1 mM EDTA, and Apo-2L was isolated from the soluble fraction by IMAC on a chelating hiTRAP column (Pharmacia) charged with nickel. The Apo-2L had a weak affinity for immobilized metal and could be eluted with low concentrations of imidazole. A final purification was obtained by cation exchange chromatography on a SP hiTRAP column (Pharmacia). Concentrations of purified Apo-2L variants were determined by absorbance measurements using an e280 of 1.4 mg−1 ml cm−1.
Sources & evidence
- ORD record
- ord-3a4a551e0db747adb87d6d1ca324e6b0
- Source dataset
- uspto-grants-2010_12
- Patent number
- US07855066B1
Reaction notes
Loading…