反应详情

The crude product obtained using dihydrochloride of Compound 18 (150 mg, 0.370 mmol) N-methylmorpholine (0.0812 mL, 0.740 mmol), 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide hydrochloride (99.3 mg, 0.370 mmol), 1-hydroxybenzotriazole monohydrate (64.9 mg, 0.481 mmol) and di(hydroxyethyl)aminoacetic acid (60.4 mg, 0.370 mmol) in a similar manner to Example 5, was purified by C-18 column chromatography (water/methanol=67/33), followed by treating with 10% hydrogen chloride-methanol solution (0.775 mL) and triturating in ethyl acetate to obtain Compound 144 (43.5 mg, 21%).

1. customwas purified by C-18 column chromatography (water/methanol=67/33)
2. additionby treating with 10% hydrogen chloride-methanol solution (0.775 mL)
3. customtriturating in ethyl acetate