Pyridoxal 5'-phosphate → Ethylenediaminetetraacetic Acid

HRID 2380186Notes

Reaction equation

HRID 2380186 reaction equation
C(O)C(N)(CO)CO.[Na+].N[C@H](C([O-])=O)CCC([O-])=O.[Na+].[CH2:21]([N:32]([CH2:37][C:38]([O-:40])=[O:39])[CH2:33][C:34]([O-:36])=[O:35])[CH2:22][N:23]([CH2:28][C:29]([O-:31])=[O:30])[CH2:24][C:25]([O-:27])=[O:26].CC1N=CC(COP(O)(O)=O)=C(C=O)C=1O.CCC(COC(C(N(CC[NH+](C)C)C)=O)(C1C=CC=CC=1)C1C=CC=CC=1)CC.[Cl-].N[C@H](C([O-])=O)CC([O-])=O>O>[CH2:22]([N:23]([CH2:28][C:29]([OH:31])=[O:30])[CH2:24][C:25]([OH:27])=[O:26])[CH2:21][N:32]([CH2:37][C:38]([OH:40])=[O:39])[CH2:33][C:34]([OH:36])=[O:35]

Participants

8

Reactant 6

HCID 1051Pyridoxal 5'-phosphateHCID 6503Tris(hydroxymethyl)aminomethaneHCID 19153X 100HCID 164551EthylenediaminetetraacetateHCID 167560Disodium glutamateHCID 5460541l-aspartic acid, sodium salt

Product 1

HCID 6049Ethylenediaminetetraacetic Acid

Reagents, catalysts & solvents 1

HCID 962WaterSolvent

Procedure

Dissolve 12.11 gm of Tris base, 50.72 gm of glutamic acid sodium salt, 1.86 gm of ethylenediaminetetraacetate (EDTA), 1.86 gm of polyvinyl pyrrolidone (PVP), 7.4 mg of pyridoxal phosphate (PP), and 50 mg of TRITON X-100 detergent (TX-100) in 1 liter of deionized water. Adjust the pH to 9.0. Add 1500 U of aspartate transaminase (AST) and mix well.

Workup

  1. additionmix well

Sources & evidence

ORD record
ord-5d006b193f9945119432a295068647b6
Source dataset
uspto-grants-1994_11
Patent number
US05366870

Reaction notes

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